CANIS ET FELIS EHRLICHIOSIS PDF

Some species A. Clinical signs are usually reported soon after tick infestation. They are mostly non-specific and consist of fever, anorexia, lethargy. Joint pain may occur. Antibodies to rickettsial infections can be detected by immunofluorescence IF test and ELISA, but cross-reactions exist between organisms of the same genus.

Author:Tushura Mikabar
Country:French Guiana
Language:English (Spanish)
Genre:Science
Published (Last):13 April 2018
Pages:216
PDF File Size:5.23 Mb
ePub File Size:5.97 Mb
ISBN:837-5-35680-712-9
Downloads:37469
Price:Free* [*Free Regsitration Required]
Uploader:Guzragore



Detection of Ehrlichia canis in domestic cats in the central-western region of Brazil. Ehrlichiosis is a worldwide distributed disease caused by different bacteria of the Ehrlichia genus that are transmitted by arthropod vectors.

Its occurrence in dogs is considered endemic in several regions of Brazil. Regarding cats, however, few studies have been done and, consequently, there is not enough data available. In order to detect Ehrlichia spp. The results obtained show that 88 The natural transmission of Ehrlichia in cats has not been fully established.

Furthermore, tick infestation was not observed in the evaluated cats and was not observed any association between age, gender and positivity of cats in both tests. The present study reports the first serological and molecular detection of E. Ehrlichiosis is a worldwide distributed disease caused by microorganisms of Ehrlichia genus that is transmitted by arthropod vectors Groves et al.

Ehrlichia spp. It is an exclusive Gram-negative intracellular parasite, located in cytoplasmic vacuoles of mature or immature hematopoietic and endothelial cells, and found in peripheral blood or tissue Unver et al.

Some domestic and wild mammals, such as canines and ruminants, are reservoirs of Ehrlichia spp. The first case of feline ehrlichiosis was described in the 80's Charpentier and Groulade, and, since then, several cases of this ehrlichiosis have been reported Stubbs et al. Ehrlichia species that naturally infect cats were not totally characterized.

Few studies in order to clarify the Ehrlichia species that infect cats were conducted, but monocytic, lymphocytic and, granulocytic inclusions were detected in cats with febrile illness and thrombocytopenia suggesting that other rickettsial species could act on feline ehrlichiosis infection. In Brazil, Ehrlichia infection was first reported by Almosny and Massard throughout the observation of ehrlichial morula in blood smears, and in it was first detected by PCR by Oliveira et al. Recently, Braga et al.

In addition, natural transmission of Ehrlichia in cats has not been fully established Amyx and Huxsoll, However, Rhipicephalus sanguineus tick demonstrates wide geographical distribution in Brazilian urban areas Labruna and Pereira, and it was previously reported in cats from the northeast region of Brazil Ferreira et al.

This study investigates the presence of Ehrlichia spp. Materials and Methods. Both products were used in accordance with the manufacturer's instructions. The presence of anti- Ehrlichia spp. Commercial fluorescein isothiocyanate-conjugated anti-cat IgG Sigma-Aldrich, Saint Louis, Missouri was used as conjugate at dilution of The antigen preparation and the IFA were executed as previously described Aguiar et al.

For IFA standardization, serums of PCR positive cats were tested to be used as positive control and a previously non-reactive serum negative control was included in each assay. Data concerning age, sex and tick parasitism in cats were used for risk factor analysis. To define age, cats with corporal mass less than 1.

The association between Ehrlichia spp. Among the evaluated cats, 93 One hundred two animals Fifty nine Age information was not available for six cats. Twenty 9. Jake CP , deposited on Genbank. According to their origin, 8 2. Eight Both gender had the same proportion Serologic tests resulted in 88 Forty-two Twenty-six Forty-five Antibody titers ranged from 40 to Two cats 2.

Twelve 5. No ticks were found in the evaluated cats. Research about the presence of Ehrlichia spp. In particular, the present study detected the highest frequency of Ehrlichia spp.

Seroprevalence detected evidence of exposure to Ehrlichia spp. However, the occurrence of cross-reactivity with other Anaplasmataceae family agents cannot be ruled out Wen et al.

Amplified DNA samples analyzed by sequencing reactions were identical to the sequences of E. The central-western region of Brazil was considered to be endemic for E. Furthermore, E. Ehrlichia is transmitted through the bite of an infected tick, mainly in tropical and subtropical regions Rodriguez-Vivas et al.

Moreover, the absence of ticks in the evaluated cats can suggest the existence of an unknown vector acting as the transmission agent in cats. Negative PCR results in seropositive cats show the lack of E. Little is known about the dynamics of E. The positive result in both tests leads to the assumption that these animals were possibly in the acute or asymptomatic phase of the infection, corresponding to the period of seroconversion. Other potential hypothesis is that these animals would be through a period of resurgence as a result of immunosuppression.

No association between age and sex was found, minimizing the effect of social behavior in the epidemiology of infection in the considered region. These results are similar to the findings of Stubbs et al. This fact is probably due to the peculiar characteristics of the area, which contribute to expose these animals to infection.

Shelter cats had a higher frequency of anti- E. Nevertheless the study contributes to other studies that suggest the role of domestic cats as reservoirs of E. We are very gratefull to A. We thank C. Cienc Rural Ann NY Acad Sci Clin Vet J Mol Biol J Wild Dis Prat Med Chir Anim Comp J Small Anim Pract J Am Vet Med Assoc Rev Bras Parasitol Vet J Vet Intern Med Charpentier F, Groulade P Report of one case of probable feline ehrlichiosis.

Bull Acad Vet Fr In: Birchard, S. J Mol Diagn Int J Syst Evol Microbiol J Feline Med Surg Rev Biotemas Am J Vet Res IBGE Cidades.

Lappin M, Feline ehrlichiosis and hemobartonellosis. In: Scherk M. Ticks Tick Borne Dis Clin Microbiol Infec Clin Microbiol Rev Vet Parasitol J Helminthol Comp Cont Educ Pract J Clin Microbiol Correspondence: D. Submitted: October 31, Approved: November 25, All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.

JURNAL SAPONIFIKASI PDF

Ehrlichiosis

Metrics details. Molecular identification of tick-borne pathogen infection in cats from Africa is scarce. The presence of bacterial Anaplasma and Ehrlichia and protozoal Babesia and Hepatozoon agents was investigated in blood samples from domestic cats from Luanda, Angola, by polymerase chain reaction and DNA sequencing. Three cats 2. The prevalence of infections with one single agent was 4. In total, six cats 5. This is the first report of A.

ANTIBIOTICOS BETALACTAMICOS EN ODONTOLOGIA PDF

Diagnosis of canine monocytotropic ehrlichiosis: an update review

Background: Molecular identification of tick-borne pathogen infection in cats from Africa is scarce. The presence of bacterial Anaplasma and Ehrlichia and protozoal Babesia and Hepatozoon agents was investigated in blood samples from domestic cats from Luanda, Angola, by polymerase chain reaction and DNA sequencing. Results: Three cats 2. The prevalence of infections with one single agent was 4.

INFORME COSO COOPERS LYBRAND PDF

Associated Content

Cats experimentally infected with E. Ehrlichia canis DNA and DNA genetically identical to the granulocytic Ehrlichia of horses, dogs, and humans in Sweden have been amplified from naturally exposed cats. Ehrlichia -like morula have been detected in mononuclear cells or neutrophils of naturally-exposed cats in the United States, Kenya, Brazil, France, Sweden, and Thailand. Clinical diagnosis has been also been based on the combination of either positive E. It is unknown how the clinically ill, naturally exposed cats described in the literature were infected.

Related Articles